The project

Nanoparticle tracking analysisThis project, which commenced in June 2012, will develop and validate methods to characterise nanomaterials in complex biological matrices.

Through collaboration with nanomaterial developers, world leading academic research groups and analytical platform producers this research project will progress current state of the art to:

  • To develop a panel of fluorescent and non-fluorescent nanomaterials characterised in the native form for their physical and chemical and optical properties.
  • To develop and validate protocols for the dispersion of nanomaterials in a range of biological matrices
  • To validate the use of high accuracy methods for the physical characterisation of nanomaterials in a serum based biological matrix
  • To establish the feasibility of using emergent image based analysis systems for the physical characterisation of nanomaterials
  • To develop traceable measurements of fluorescent nanomaterial quantum yield in a serum based biological matrix
  • To develop and validate methods for the simultaneous physical and chemical characterisation of nanomaterials in a complex cell based biological matrix.
  • Establish the measurement uncertainty associated with nanomaterial fluorescence in nanobiotechnology applications

Critical review of the determination of photoluminescence quantum yields of luminescent reporters

Analytical and Bioanalytical Chemistry, January 2015, Volume 407, Issue 1, pp 59-78 C. Würth, D. Geißler, T. Behnke, M. Kaiser, U. Resch-Genger Published October 2014 DOI: 10.1007/s00216-014-8130-z Link to publication: http://link.springer.com/article/10.1007%2Fs00216-014-8130-z Abstract: A crucial variable for methodical performance evaluation and comparison of luminescent reporters is the photoluminescence quantum yield (Φ pl). This quantity, defined as …

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